J. Bevilaqua, J. Pinto, L. M. Lima
Sep 1, 2004
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0
Influential Citations
9
Citations
Journal
Biochemical Engineering Journal
Abstract
Abstract The last step of the production of 4-ethyl-(2-(1,3-dioxo-1,3-dihydro-2-isoindoylyl))-phenoxy acetic acid (LASSBio 482), designed to act as an anti-asthma drug, was performed by enzymatic hydrolysis of the methyl ester. Reactions were performed in a three-phase system with a solid biocatalyst (Lipozyme ® RM IM), an organic solvent phase (ethyl acetate) and an aqueous phase (saturated Na 2 CO 3 solution). In order to optimize the reaction conditions, experimental design techniques were used. The variables studied were the amount of enzyme, the reaction temperature and the total volume of saturated Na 2 CO 3 solution. The experimental results were analysed statistically and the main effects of the variables were computed and analysed. Dynamic experiments were then performed under optimised reaction conditions for different initial enzyme concentrations (0.5, 0.9 and 1.4 U H Lipozyme /mL solvent). An empirical nonlinear model was then proposed to describe the process, and experimental data were used to estimate the model parameters. The optimised reaction conditions were 20 mg Lipozyme ® (0.9 U H /mL solvent ), 5.0 mL Na 2 CO 3 (sat), a temperature of 40 °C and batch reaction time of 6 h. The results showed that enzymatic hydrolysis is a promising technique for the synthesis of the desired product.