B. Angelova, P. Fernandes, A. Cruz
Dec 1, 2005
Citations
0
Influential Citations
17
Citations
Journal
Enzyme and Microbial Technology
Abstract
The use of organic solvent-based media for microbial 9-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3:1, v/v) containing 1.0 g L −1 AD. The results suggest that in these conditions the reaction of � 1 -dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v).