Deng Kai
2013
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Chinese Journal of Gastroenterology and Hepatology
Abstract
Objective To investigate the effect of gastrin on rats model of gastric mucosa with N-methyl-N'-nitro-Nnitrosoguanidine ( MNNG) . Methods 24 Wistar rats were divided into 4 groups: blank control group ( group A,n = 6) was given water alone,gastrin group ( group G,n = 6) was given pentagastrin 300 μg / kg for 90 days,MNNG group ( group M,n =6) was given MNNG water alone,gastrin and MNNG group ( group M + G,n = 6) were given MNNG water and pentagastrin 300 μg / kg for 90 days. After 90 days,rats were euthanized. Gastric mucosa was evaluated histologically. The expression of bcl-2 and ki-67 were examined with immunohistochemical staining technique. Results There were difference in gastric mucosal layer thickness among 4 groups ( P 0. 05) ,compared with group A and group M,mucosal thickness in group G and group M + G were increased. Compared with group A,expression of bcl-2 and ki67 in group G,group M and group M + G were higher,the raise was statistically significant ( P 0. 05) . Conclusion Gastrin can promote the growth of the gastric mucosa and increase the expression of ki-67 and bcl-2,it suggests that gastrin alone may contribute to malignant change through promoting cell proliferation and inhibiting apoptosis.