Souravh Bais, Y. Prashar
Feb 1, 2015
Citations
1
Influential Citations
12
Citations
Quality indicators
Journal
Research Journal of Phytochemistry
Abstract
The aim of the present study was to investigate the protective effects of amentoflavone (AF), isolated from six species of Juniperus (AF1-AF6) (Cupressaceae) Juniperus communis L. (AF1), Juniperus wallichiana L. (AF2), Juniperus indica L. (AF3), Juniperus macropoda L. (AF4), juniperus recurva L. (AF5) and Juniperus polycarpos L. (AF6)] against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The experiment was set and designed as per method available in literature. Amentoflavone was isolated from aerial parts of Juniperus species (above six) and authenticated by TLC, IR, HPLC and physicochemical parameters. The two concentration of isolated AF (A = 250 μL, B = 500 μL) were used to assessed its protective effects against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The measurements of different antioxidant parameters like, CAT, SOD and GPx, LPO and GSH levels were performed under each set extracts. Juniperus communis L. (AF1B) (500 μL) and Juniperus indica L. (AF3B) (500 μL) were the most significant one on CAT (28.35±2.980 and 27.73±1.580 AU gG1 Hb), GPx (128.53±3.10 and 41.7±1.420 μg gG1 Hb) and SOD (1193.5±19.7 and 1532.6±17.60 U gG1 Hb) enzyme systems of erythrocytes. Among plant extracts, Juniperus communis L. (AF1B) showed the highest activities on CAT (13.79±1.832 AU mgG1 protein) and SOD (820±14.50 U mgG1 protein) while Juniperus indica L. (AF3B) showed significant effects on Gpx (152.72±3.70 U mgG1 protein), GSH (25.93±1.560 μg mgG1 protein) and LPO (10.54±2.90 nmol mgG1 protein) enzyme systems of leucocytes. The study concludes that isolated fractions of AF from Juniperus species (among six species), has a potential source of natural antioxidants for treatment and prevention of diseases in which oxidative stress takes place.