Chen San-feng
2012
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Journal
China Oils and Fats
Abstract
12-methyltetradecanoic acid(12-MTA,anteiso-C15 ∶ 0) possesses notable antitumor activity.The spore-producing bacterium X1 isolated from severe saline-alkali soil was taken as research object,and its taxonomic position was determined by 16S rDNA-based phylogenetic analysis and phenotypic characteristics.The total fatty acid methyl ester derivatives of X1 were prepared through cell lysis,fat saponification,HCl-catalyzed methyl esterification and extraction,and their compositions were analyzed by GC-MS.The net weight of total fatty acid methyl ester derivatives of X1 was determined by weighing method.The results showed that the strain X1 was a member of genus Bacillus,and the 16S rDNA similarity data showed it should be a novel species within the genus Bacillus.GC-MS analysis determined that fatty acids of X1 mainly contained four kinds of fatty acids which were iso-C14 ∶ 0,C14 ∶ 0,iso-C15 ∶ 0 and anteiso-C15 ∶ 0,of which anteiso-C15 ∶ 0(12-MTA) was 76.2% of the total fatty acid content of X1 and 4.47% of X1 dry weight.