A. Beckett, M. Mitchard, A. A. Shihab
May 1, 1971
Citations
0
Influential Citations
41
Citations
Journal
Journal of Pharmacy and Pharmacology
Abstract
Isomethadone and normethadone are metabolized by microsomal preparations of guinea‐pig liver to yield 2‐ethyl‐1,4‐dimethyl‐3,3‐diphenyl‐1‐pyrroline and 2‐ethyl‐1‐methyl‐3,3‐diphenyl‐1‐pyrroline respectively. The structures of the pyrrolines were established (by comparison with the pyrroline derived from methadone) by thin‐layer chromatography and by infrared and nuclear magnetic resonance spectral data. Methadone, isomethadone and normethadone are also metabolized to the corresponding N‐oxides. A gas chromatographic procedure for the quantitative determination of unchanged drugs, cyclic metabolites and N‐oxides of methadone, isomethadone and normethadone in microsomal homogenates is described. The N‐oxides were reduced before analysis.