Na Liu, Xige Wang, Hongqiang Liu
Nov 15, 2019
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Influential Citations
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Quality indicators
Journal
Rapid communications in mass spectrometry : RCM
Abstract
Rationale Erianin, a bioactive component isolated from Dctidrobium chrysotoxum Lindl, was demonstrated to have many biological properties relevant to cancer prevention and therapy. However, the metabolic profiles of erianin remain unknown. This study was carried out to investigate the metabolic profiles of erianin in rats and humans. Methods Erianin was orally administered to rats at a single dose of 50 mg/kg. The urine and bile samples were collected. For in vitro metabolism, erianin was co-incubated with rat or human hepatocytes at 37 o C for 2 h. The samples from incubations and rat were analyzed by liquid chromatography combined with electrospray ionization high resolution mass spectrometry. The data were processed by MetWorks software. The structures of the metabolites were proposed by comparing the mass spectra with that of the parent compound. Results A total of twenty-four metabolites were detected in vitro and in vivo, including seven phase I and eighteen phase II metabolites. The phase I metabolic pathways of erianin were hydroxylation, demethylation and dehydrogenation. Erianin undergoes metabolic activation to form reactive metabolites quinoids intermediates, which were further trapped by glutathione or N-acetyl-cysteine. The phase II metabolic pathways were glucuronidation, glutathione and N-acetyl-cysteine conjugation. Conclusions The present study provides an overview pertaining to the in vitro and in vivo metabolic profiles of erianin, which is indispensable for us to understand the efficacy and safety of erianin, as well as the herb medicine D. chrysotoxum.