S. Angal, P. Dean
Dec 15, 1978
Citations
0
Influential Citations
34
Citations
Journal
FEBS Letters
Abstract
Human serum albumin is a major contaminant in the isolation of many plasma proteins. It constitutes about 55% of the total plasma protein and is responsible for non-specific binding to many affinity columns. The use of Blue Dextran to abstract albumin from plasma was first reported by Travis and Pannell in 1974 [I] . The efficiency of the system was improved [2] by the use of the chromophore of Blue Dextran, viz. Cibacron Blue, fig.1, attached directly to agarose. We have already reported the influence of the matrix on the interaction of immobilized Cibacron Blue with albumin [3] . In this paper we describe the effect of pH on the adsorption of plasma by Cibacron Blue-Sepharose.