Zexiang Wang, Song Hu, H. Bao
Jul 24, 2020
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Journal
Journal of the science of food and agriculture
Abstract
BACKGROUND Sulfamethazine (SMZ), a veterinary drug widely used in animal husbandry, is harmful to human health when excess residues are present in food. In this study, a fast, reliable, and sensitive immunochromatographic assay (ICA) was developed on the basis of the competitive format by using time-resolved fluorescent nanobeads (TRFN) as label for the detection of SMZ in egg, honey, and pork samples. RESULTS Under optimized working conditions, this method had limits of detection of 0.016, 0.049, and 0.029 ng mL-1 and corresponding linear ranges of 0.05-1.00, 0.05-5.00, and 0.05-1.00 ng mL-1 in egg, honey, and pork samples, respectively. The recovery experiments showed that the average recoveries ranged from 90.5%-113.9%, 82.4%-112.0%, and 79.8%-93.4% with corresponding coefficients of variation of 4.1%-11.7%, 7.5%-11.5%, and 4.8%-8.7% for egg, honey, and pork samples, respectively. The developed TRFN-ICA was also systematically compared with HPLC-MS/MS by analyzing 45 actual samples from egg, honey, and pork. CONCLUSION Overall, the developed TRFN-ICA had high reliability and excellent potential for the ultrasensitive detection of SMZ for food safety monitoring, also providing a universal platform for the on-site detection of other targets. This article is protected by copyright. All rights reserved.