C. Smith, F. Snyder, L. Fontenelle
Jul 15, 1974
Citations
0
Influential Citations
8
Citations
Journal
Biochemical pharmacology
Abstract
Abstract Procedures are described by which the apparent rates of 23 enzymes of purine metabolism can be calculated from data obtained using intact Ehrlich ascites cells incubated in vitro, and these have been applied to the study of the effects of certain purine analogs and derivatives. Nebularine (9-β- d -ribofuranosyl purine), 7-deazanebularine (7-β- d -ribofuranosyl pyrrolopyrimidine) and related compounds inhibit nucleotide synthesis from radioactive adenine, guanine and hypoxanthine in Ehrlich ascites tumor cells in vitro, and also inhibit phosphoribosyl pyrophosphate accumulation in cells incubated with glucose. Nebularine and 7-deazanebularine inhibit the growth of cultured leukemia RPMI 6410 cells.