N. Praveen, H. N. Murthy, I. Chung
Mar 1, 2011
Citations
3
Influential Citations
39
Citations
Journal
Industrial Crops and Products
Abstract
Abstract Gymnema sylvestre is an important medicinal plant which bears bioactive compound namely gymnemic acids. The present work deals with optimization of cell suspension culture system of G. sylvestre for the production of biomass and gymnemic acid and we investigated effects of macro elements (NH 4 NO 3 , KNO 3 , CaCl 2 , MgSO 4 and KH 2 PO 4 – 0.0, 0.5, 1.0, 1.5 and 2.0× strength) and nitrogen source [NH 4 + /NO 3 − ratio of: 0.00/18.80, 7.19/18.80, 14.38/18.80, 21.57/18.80, 28.75/18.80, 14.38/0.00, 14.38/9.40, 14.38/18.80, 14.38/28.20 and 14.38/37.60 (mM)] of Murashige and Skoog medium on accumulation of biomass and gymnemic acid content. The highest accumulation of biomass (165.00 g l −1 FW and 15.42 g l −1 DW) was recorded in the medium with 0.5× concentration of NH 4 NO 3 and the highest production of gymnemic acid content was recorded in the medium with 2.0× KH 2 PO 4 (11.32 mg g −1 DW). The NH 4 + /NO 3 − ratio also influenced cell growth and gymnemic acid production; both parameters were greater when the NO 3 − concentration was higher than that of NH 4 + . Maximum biomass growth (159.72 g l −1 of FW and 14.95 g l −1 of DW) was achieved at an NH 4 + /NO 3 − ratio of 7.19/18.80, and gymnemic acid production was also greatest at the same concentration of NH 4 + /NO 3 − ratio (11.35 mg g −1 DW).