A. M. Skogoreva, O. Manzhurina, O. Popova
Jan 9, 2020
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Quality indicators
Journal
IOP Conference Series: Earth and Environmental Science
Abstract
In order to improve the immune response to salmonellosis in vaccinated calves, we tried applying the 0.01 % solution of miramistin combined with an inactive emulsified vaccine. Timogen was used as the control medicine because it is well known for its immunomodulatory properties regulating the cellular and the humoral immunity. The research was carried out at a large farm in Lipetskaya oblast that has a high salmonellosis incidence rate among calves. This research uses the epizootological, the clinical, the immunological, the hematological, and the molecular genetic methods. We ascertained that the application of the 0.01% solution of miramistin and timogen to calves for the purposes of salmonellosis etioprophylaxis combined with an inactive emulsified vaccine promoted high cellular immunity. These medicines increased the levels of lymphocytes and their T-cells by 7.0% and 12.5%; their B-populations by 2.8% and 5.1%, the neutrophil phagocytic activity by 5.9% and 13.0%, the phagocytic number by 7.2% and 15.0%, the phagocytic index by 7.0% and 18.0% respectively. The improvement of humoral immunity factors comprised 3.0% and 7.2% for blood serum bactericidal activity; 3.0% н 4.3% for blood serum lysozyme activity; and 1.7% and 2.0% for blood serum complement activity. The O-agglutinins and H-agglutinins to salmonella antigen increased by 1.5 and 2 times respectively, and the disease rate decreased by 40.0% and 80.0%, while promoting the survival rate by 80.0% and 87.0% respectively, which shows that the 0.01% solution of miramistin has better immunomodulatory properties.