P. B. Crowley, Ciara Kyne, W. Monteith
Oct 3, 2012
Citations
1
Influential Citations
104
Citations
Quality indicators
Journal
Chemical communications
Abstract
Fluorine-containing amino acids are valuable probes for the biophysical characterization of proteins. Current methods for (19)F-labeled protein production involve time-consuming genetic manipulation, compromised expression systems and expensive reagents. We show that Escherichia coli BL21, the workhorse of protein production, can utilise fluoroindole for the biosynthesis of proteins containing (19)F-tryptophan.