M. Sprinzl, F. Von der Haar, E. Schlimme
Feb 1, 1972
Citations
0
Influential Citations
34
Citations
Journal
European journal of biochemistry
Abstract
5-Iodocytidine (io5C) is incorporated into tRNAPhe-A73 as well as into tRNAPhe-A73-C74 using tRNA nucleotidyl transferase. The tRNAPhe-A73-C74-io5C75-A76 and tRNAPhe-A73-io5C74-io5C75-A76, respectively, thus obtained can be aminoacylated by the corresponding synthetase exhibiting the same Km but a slightly lowered V compared to tRNAPhe-A73-C74-C75-A76. An analytical method is presented which enables quantitative determination of the 3′ terminal nucleosides.