K. Van den Berg, I. Betel
1973
Citations
0
Influential Citations
57
Citations
Quality indicators
Journal
Experimental cell research
Abstract
Abstract The transport of 2-aminoisobutyric acid has been studied in stimulated rat lymphocytes. The capacity to accumulate 2-aminoisobutyric acid increased twofold after a 5 min incubation with phytohaemagglutinin (PHA) and concanavalin A (conA). It reached a maximum level which was 6–8 times the level of the control culture after 24 h of incubation with PHA. The early increased transport induced by conA could not be attributed to a decreased efflux, increased exchange diffusion or passive diffusion but reflected an increased rate of active influx. Kinetic analysis showed an increase in the maximum velocity of uptake ( V max ) while no effect was observed on the apparent affinity ( Km ). The increased uptake was dependent on protein synthesis and only partly dependent on the generation of metabolic energy. It is concluded that concanavalin A has no direct effect on the binding of 2-aminoisobutyric acid to the carrier and on the mobility or the number of carriers.