Wang Wei
2006
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0
Influential Citations
1
Citations
Quality indicators
Journal
Journal of Beijing University of Traditional Chinese Medicine
Abstract
Objective To study the influence of typhaneoside on the proliferation of human umbilical arterial smooth muscle cells(HUASMC).Methods ① HUASMC were primary cultured with tissue-sticking method and determined by α-actin detection.The third to fourth generations of cells were put into the experiment.② The highest concentration of typhaneoside was 1.00×10-5 mol/L and then diluted in multiple proportions into 11 concentration groups,and a blank control group was established.After the stimulations were given to different groups for 24,48 and 72 hours,the toxicity of typhaneoside were detected with MTT assay.③ The highest concentration of typhaneoside was 1.00×10-6 mol/L and then diluted in 1/4 isobaric proportion into 6 concentration groups,and a blank control group was established. After the stimulations were given to different groups for 24,48 and 72 hours,the influence of typhaneoside on the proliferation of HUASMC was observed with MTT assay.Results ① The cellular staining was positive under the microscope.② Typhaneoside was not toxic to HUASMC in the concentration ranges from 9.77×10-9 mol/L to 1.00×10-5 mol/L after 24,48 and 72 hours.③ Typhaneoside did not inhibit the proliferation of HUASMC in the concentration ranges of 9.77×10-10 mol/L to 1.00×10-6 mol/L after 24 or 48 hours,but inhibited significantly the proliferation of HUASMC after 72 hours in the concentration ranges of 1.56×10-8 mol/L to 2.5×10-7 mol/L.The effect was increased along with the dose increase but there was no significant difference.The inhibitory rate reached 9.91% to 14.99%.Conclusion Typhaneoside has an inhibitory effect on the proliferation of HUASMC with a wide range of effective dose but the effect is started slowly.