M. Klingenberg, K. Grebe, H. Heldt
May 11, 1970
Citations
1
Influential Citations
67
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract Bongkrekic acid (BA) is shown to inhibit the adenine nucleotide (AdN) exchange in rat liver mitochondria. BA inhibits only above 20°, once applied at this temperature also at 0°. Quantitative evaluation of the inhibition of the translocation rate gives the number of inhibitor sites NI = 0.2 to 0.3 μmoles/g protein and dissociation constant KI = 2 × 10−8M. Similar results are obtained for inhibition of respiration. It is shown that, in accordance with the inhibition of translocation, BA inhibits phosphorylation only of exogenous but not of endogenous AdN, similar to atractyloside (AT).