Austin L. Shug, Edith Lerner, Charles E. Elson
May 7, 1971
Citations
1
Influential Citations
163
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
The inhibitory effect of oleic acid on the 32Pi-ATP exchange activity and 14C-ADP translocation through the mitochondrial membrane can be reversed by substrates preferentially utilizing CoA and thereby preventing acylation of the fatty acid. By contrast the inhibition produced by oleoyl CoA can be reversed only when carnitine is added to augment oxidation of the CoA ester. The slow rate of respiration and sluggish response to ADP in oleoyl CoA treated mitochondria is overcome by the uncoupler, salicyl-anilide XIII. Inhibition of translocation of adenine nucleotides through the inner mitochondrial membrane by oleoyl CoA could regulate metabolism by inducing a transition from state 3 to state 4 respiration.