Wang Xun
2008
Citations
0
Influential Citations
0
Citations
Quality indicators
Journal
Medical Journal of West China
Abstract
Objective To investigate the inhibition effect of seratrodast on the hypersecretion of airway mucus in rats with chronic obstructive pulmonary disease(COPD) and its molecular mechanism.Methods Forty male SD rats were randomly divided into four groups:blank control group,drug control group,model group,and drug intervention group.The expression of mucus gene(MUC5AC) and mucin in bronchial tissues was examined by histochemical analysis and alcian blue-periodic acid schiff(AB-PAS) staining,respectively.The mRNA expression of MUC5AC was measured by reverse transcription-polymerase chain reaction(RT-PCR).The signal protein p38 and P-erk expressions in lung tissue were detected by Western blot.Results The overexpression of MUC5AC,mucin,MUC5AC mRNA and signal protein p38,P-erk was seen in model group as compared with control group.The expression of MUC5AC,mucin,MUC5AC mRNA and signal protein p38,P-erk was gradually decreased in the drug intervention group(P0.01) in a dose-dependent manner.Conclusion Seratrodast can decrease the expression of p38,P-ERK and suppress mucus hypersecretion in rat airways.