P. Sane, U. Johanningmeier, A. Trebst
Dec 1, 1979
Citations
1
Influential Citations
41
Citations
Journal
FEBS Letters
Abstract
Dicyclohexylcarbodiimide (DCCD) is a well known inhibitor of photophosphorylation [ 1,2] and oxidative phosphorylation [3]. It prevents ATP formation and also suppresses electron flow, the latter rate being restored by the addition of an uncoupler [4]. This shows that DCCD behaves like a classical energy transfer inhibitor. Its mode of action is due to a binding onto an amino acid of the base piece or proton channel (CFo or F,) of the coupling system, which indirectly affects the rate of electron flow [5]. In the photosynthetic electron flow system of chloroplasts however, it is known that DCCD at somewhat higher concentrations has another effect on electron flow in addition to that of an energy transfer inhibitor. The basal (not coupled) rate of electron flow is sensitive to the addition of DCCD but the reversal of the inhibition of the coupled rate by an uncoupler is not complete [ 11. This additional effect of DCCD has not been analysed yet in detail, let alone localized at a specific site [6]. By using recent progress in artificial donor and acceptor