L. Gauthier, J. J. Diwan
Apr 27, 1979
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0
Influential Citations
28
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Summary Dicyclohexylcarbodiimide (DCCD), which has been reported to react covalently with a subunit of the ATP synthase complex involved in proton translocation, decreases the rate of unidirectional K+ flux into rat liver mitochondria in the presence of the respiratory substrate, succinate. In contrast, oligomycin does not affect the rate of K+ influx. At concentrations which completely block ADP-stimulated respiration, DCCD increases the apparent Km for K+ of the K+ transport mechanism, while having little effect on the Vmax of K+ influx. Inhibition of K+ influx by DCCD is observed over the whole pH range tested from 6.8 to 8.0. A slight, but reproducible, decrease in the pH-dependence of K+ influx is observed in the presence of DCCD.