H. Wolf, J. Schlepper, V. Steinijans
Jun 15, 1980
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Quality indicators
Journal
Biochemical pharmacology
Abstract
Abstract The effect of clanobutin {4-[ p -chloro- N -( p -methoxyphenyl-)benzamido]butyric acid} on gluconeogenesis from lactate + pyruvate (1.6 + 0.2 mmoles/1) as precursors in isolated perfused liver of fasted rats was investigated. Glucose production was dose dependent inhibited up to a maximum of 81 ± 3 per cent; the half-maximum concentration of clanobutin was 0.33 ± 0.04 mmoles/1. Buformin and phenformin, respectively, used as references showed no effect on gluconeogenesis under the given experimental conditions. Oxygen uptake was not inhibited by clanobutin at concentrations up to 0.14 mmoles/1. At higher concentrations, the inhibitory effect was smaller than observed for comparable buformin doses. According to our results, clanobutin appears to be a more potent and probably more specific inhibitor of gluconeogenesis than the therapeutically used biguanides—at least in the isolated perfused rat liver.