R. Tamizhselvi, P. Moore, M. Bhatia
May 1, 2008
Citations
2
Influential Citations
73
Citations
Quality indicators
Journal
Pancreas
Abstract
Objectives: The present study investigated whether chemokines are involved in hydrogen sulfide (H2S)-associated pathogenesis of acute pancreatitis and associated lung injury. Methods: We have examined the effect of DL-propargylglycine, a cystathionine &ggr;-lyase inhibitor, on the synthesis of CC chemokine monocyte chemotactic protein 1, Regulated upon Activation, Normal T-cell Expressed, and Secreted, and macrophage inflammatory protein-1&agr; (MIP-1&agr;), and CXC chemokine MIP-2 in an in vitro and in vivo model of cerulein-induced acute pancreatitis and associated lung injury. In addition, the pancreatic acinar cells were treated with H2S donor drug, sodium hydrosulfide. The expression ofthese chemokines in the pancreatic acini, pancreas, and lungs wasdetermined by quantitative real-time reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. Results: After treatment with DL-propargylglycine, reverse transcriptase polymerase chain reaction, and enzyme-linked immunosorbent assay demonstrated down-regulation of cerulein-induced increase in monocyte chemotactic protein 1, MIP-1&agr;, and MIP-2 expression but had no apparent effect on Regulated upon Activation, Normal T-cell Expressed, and Secreted expression. Conclusions: These results suggest that the proinflammatory effect of H2S may be mediated by chemokines.