C. Young, P. Robinson, B. Sacktor
Aug 1, 1963
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0
Influential Citations
122
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Quality indicators
Journal
Biochemical pharmacology
Abstract
Abstract The antibiotic, acetoxycycloheximide, markedly inhihits the incorporation of 14C-amino acids into tissue proteins and Ehrlich ascites cells. Inhibition of inorganic 52P incorporation into phosphoprotein is observed, whereas incorporation into acid-soluble compounds, RNA, and phospholipid is unaffected. The block of protein synthesis accompanies a syndrome characterized by vomiting, diarrhea, gradual hypotension, and death. The biochemical changes associated with this syndrome are progressive acidosis and elevation in the levels of plasma nonprotein N, urea N, amino acid N, potassium, inorganic phosphate and sulfate. The amount of plasma amino acid N increases at least three-fold within 3 hr of injection of drug; all the amino acids normally found in plasma are increased in concentrations. The antibiotics, actidione and puromycin, in doses several orders of magnitude greater than those of acetoxy-cycloheximide, also inhibit protein synthesis in the intact animal and induce a similar syndrome. The suggestion is made that this syndrome will prove to be typical of that induced by protein synthetic blockade in the intact animal.