A. Abdel-Rahman, O. Ali, E. Pedersen
Nov 25, 1996
Citations
0
Influential Citations
15
Citations
Journal
Tetrahedron
Abstract
Abstract 5′- O -(4,4′-dimethoxytrityl)-5-methyl- N 4 -(1-pyrenylmethyl)cytidine( 5 ) was prepared by reaction of 1-pyrenylmethylamine with an appropriate protected 4-(1,2,4-triazolyl)thymidine derivative which was synthesized from 5- O -DMT protected thymidine by acetylation with acetic anhydride and subsequent reaction with triethylamine, 1,2,4-triazole and POCl 3 . Stabilities are reported for DNA duplexes, three-way junctions and triplexes when 5 is inserted. Most interestingly, the three-way junctions are stabilized when 5 is used for insertion into the junction region. This break-through for recognizing the foot of a stem could have far-reaching importance because new targets for antisense oligos is now rendered possible on intact secondary structures.