L. Fen
2014
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0
Influential Citations
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Citations
Quality indicators
Journal
Chinese Journal of Experimental Traditional Medical Formulae
Abstract
Objective: The aim of this study was to investigate the interaction of( E)-2- [2-( 4-methoxybenzylidene) hydrazinyl]-4-phenylthiazole( MHP) and human Albumin( HSA),in order to provide experimental basis for revealing the mechanism of interaction between MHP and HSA. Method: The impactof MHP on HSA conformation was investigated by using synchronous fluorescence spectroscopy,based on SternVolmer equation and the theory of non-radiative energy transferring. Result: The experiment result showed that MHP can lead to the enhancement of HSA fluorescence,indicating energy transferring between them. And the mechanism of energy transferring is that MHP and HSA formed complexes. Fluorescence enhancement( sensitization effect) mainly resulted from the-energy transferring of dipole-dipole effect between donor and receptor. Conclusion: The results of the energy transferring were the fluorescence quenching of endogenous chromophoric group,and the fluorescence sensitization of exogenous chromophoric group,and the sensitization constant was calculated as- 2. 620 8 × 104. Synchronous fluorescence spectra showed that the interaction between HSA and HA could cause the HSA conformation change,implying the binding sites closer to tryptophan.