M. Palumbo, E. Jaeger, S. Knof
Sep 22, 1980
Citations
1
Influential Citations
10
Citations
Quality indicators
Journal
FEBS Letters
Abstract
The biological action of the gastrin hormones is confined to the C-terminal tetrapeptide amide TrpMet-Asp-Phe-NH2 [2-51. The physiological role of the remaining peptide sequences is, however, still obscure, as no clear evidecce for a specific function has been found. Among the various possibilities the -(G~u)~sequence common to big gastrin, little gastrin and minigastrin could be suited for the binding of metal ions such as calcium or magnesium. To test this hypothesis we studied the interaction of Nle”-minigastrin I (Nle”-HG-13) with Ca”. The investigation was carried out in trifluoroethanol as the solvent, which mimics the lipophilic environment of a membrane [6], where the binding process could occur.