J. Hoebeke, G. Nijen, M. Brabander
Mar 22, 1976
Citations
2
Influential Citations
364
Citations
Quality indicators
Journal
Biochemical and biophysical research communications
Abstract
Abstract Oncodazole (R 17934), methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl] carbamate (I), a new synthetic drug with anti-tumoral activity, inhibits the polymerization of rat brain tubulin in vitro . It has no depolymerizing effect on preformed microtubules in vitro . Binding studies by means of molecular sieving and equilibrium dialysis indicates that the drug binds to purified rat brain tubulin in a mole to mole ratio. Finally the drug competitively inhibits colchicine binding to purified rat brain tubulin. From these results the conclusion may be drawn that oncodazole is a true microtubule inhibitor.