Dingyi Yu, Yong Zhang, B. Liu
May 15, 2008
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0
Influential Citations
67
Citations
Journal
Macromolecules
Abstract
A simple method for protein detection and quantification has been developed by taking advantage of the aggregation-induced fluorescence change of anionic water-soluble conjugated polymers. These polymers contain charged carboxylate groups and a π-electron delocalized optically active backbone composed of fluorene segments and 2,1,3-benzothiadiazole (BT) units. The polymers were synthesized through the Suzuki coupling between 2,7-[bis(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-9,9-bis(3-(tert-butyl propanoate))]fluorene, 2,7-dibromo-9,9-bis(3′-(tert-butylpropanoate))fluorene, and 4,7-dibromo-2,1,3-benzothiadiazole, which was followed by treatment in trifluoroacetic acid to afford the functional carboxylic acid groups. P1-BTx and P2-BTx refer to the neutral precursor polymers and the anionic water-soluble polymers, respectively. The subscript in P1-BTx and P2-BTx (x = 7.5, 15, 30) refers to the molar percentage of BT units in the polymer backbone, which is 7.5%, 15%, and 30%, respectively. Both the optica...