R. Kellogg, B. V. Keulen
1986
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Abstract
The enzyme 3-phosphoglyceraldehyde dehydrogenase has at the active site an active cysteine (Cys-149) and a histidine residue (His-176). In the presence of the coenzyme NAD+ the substrate 3-phosphoglyceraldehyde is oxidized to an acyl phosphate (Eq. (1)). NADH is produced. A thioacylderivative of Cys-149 is first formed and is Open image in new window subsequently converted to the acyl phosphate at another site on the enzyme. A hemithioacetal intermediate with Cys-149 is involved in the oxidative step. The overall sequence is summarized in Scheme 1 [1].