I. Carrico, Brian L. Carlson, C. Bertozzi
Apr 22, 2007
Citations
8
Influential Citations
382
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Quality indicators
Journal
Nature chemical biology
Abstract
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.