Wen Jin-kun
2008
Citations
0
Influential Citations
1
Citations
Quality indicators
Journal
Chinese Journal of New Drugs
Abstract
Objective:To investigate the effect of inulicin on the expression of cyclo-oxygenase-2(COX-2) and inducible nitric oxide synthase(iNOS) in the hippocampus of Alzheimer disease(AD) rats,and to explore the molecular mechanism underlying inulicin effect.Methods:Aβ25-35 was microinjected into the hippocampus to induce AD in Sprague-Dawley(SD) rats.Rats(n=10) were pretreated with intragastric inulicin(26 mg·kg-1·d-1),twice a day,for 21 days(3 days before and 18 days after AD induction).Morris water maze was used to examine spatial and memory performance.The COX-2 and iNOS proteins in the hippocampus were detected by immunohistochemistry and Western blot analysis.Results:The spatial and memory were impaired in AD rats;they showed longer escape latency and less frequency trying to find the platform(P0.05).Inulicin significantly decreased the average escape latency at days 2 to 4 in Morris water maze test(P0.05).The expressions of COX-2 and iNOS in the hippocampus of AD rats were increased(P0.05),and the increased expressions were significantly inhibited by inulicin(P0.05).Conclusion: Inulicin has a protective effect in AD rats;this effect is related to the suppression of COX-2 and iNOS expressions.