Chia-pin Chang, D. Nagel, M. Zaghloul
Jan 25, 2011
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Journal
Journal of Photochemistry and Photobiology A-chemistry
Abstract
Abstract Resorufin is a fluorescent reporter molecule, the end product in a variety of biochemical analyses. In such assays, excitation sources with high intensity are desirable for fast and precise results. However, high intensity sources bleach resorufin. We report quantitative bleaching rates for resorufin subjected to green excitation over an irradiance range of 5 to about 600 mW/cm2.