P. Kuhn, D. A. Smith
Mar 1, 1979
Citations
0
Influential Citations
26
Citations
Journal
Physiologial Plant Pathology
Abstract
Abstract The metabolic conversion of the phytoalexin kievitone to kievitone hydrate was found to occur in liquid cultures of Fusarium solani f. sp. phaseoli and in agar supporting growth of this fungus. Isolation of kievitone hydrate from agar surrounding fungal colonies suggested the possible involvement of an extracellular enzymic system in the alteration. Although a basal level of enzymic activity was detected in control culture filtrates, this was enhanced by kievitone pretreatment. The conversion of kievitone to kievitone hydrate was apparently mediated by a single enzyme, designated “kievitone hydratase”. Partial purification of this enzyme was accomplished by desalting through Sephadex G-25, concentration on a “Minicon” B-15 macrosolute concentrator and finally by elution from a column of Sephadex G200; the mol. wt was estimated to be c. 175 000. Kievitone hydratase also detoxified phaseollidin, producing a more polar metabolite (M+342), apparently a hydrated derivative.