W. Taylor
Dec 20, 1958
Citations
0
Influential Citations
2
Citations
Journal
Nature
Abstract
GIRARD'S reagent T (trimethylammonium hydrazide chloride)1 is extensively used for the separation of oxo-steroids from other steroids and particularly for purification of 17-oxo-steroids in extracts of acid- or enzyme-hydrolysed urine2. The method employed usually involves dissolving the material and Girard reagent in glacial acetic acid, and either heating the mixture for periods of up to 1 hr. or allowing it to stand overnight at room temperature. During this procedure destruction of labile steroids and some acetylation of hydroxy-steroids tend to occur (ref. 3 and unpublished observations).