M. Stulberg
Mar 10, 1967
Citations
0
Influential Citations
88
Citations
Quality indicators
Journal
The Journal of biological chemistry
Abstract
Abstract Phenylalanyl ribonucleic acid synthetase has been isolated from Escherichia coli and is 93% pure as determined by analytical centrifugation and gel electrophoresis. The procedure is adaptable to large scale preparation. No other aminoacyl-RNA synthetases are present in the purified preparation. The molecular weight of the enzyme is 181,000, with an s20,w of 8.6. Optimal reaction conditions have been determined with highly purified phenylalanine transfer RNA that exhibits a Km of 2.6 x 10-7 m. The enzyme is stable when stored at 4°.