Qing-Tao Shen, Xiulan Chen, Caiyun Sun
Aug 1, 2004
Citations
0
Influential Citations
0
Citations
Journal
Journal of Zhejiang University Agriculture and Life Sciences
Abstract
The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination peptides, identified these metabolites as three peptaibols named Trichokonin VI, VII and VIII, and their sequences were confirmed with NMR.