Song Shui
2011
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Journal
Microbiology, China
Abstract
(R)-3-Quinuclidinol is an important chiral building block for the synthesis of a variety of pharmaceutical.A strain which is able to transforming quinuclidinone into reductase(R)-3-Quinuclid inol was isolated from soil by using quinuclidinone as sole carbon sourse in the screening medium.The physiological and bio-chemical identification and 18S DNA sequense analysis revealed that this strain belongs to Rhodotorula mucilaginosa and was named as R.mucilaginosa X15.The present results in-dicated that the optimal conditions for the transforming reaction with resting cells of R.mucilaginosa X15 were 3-quinuclidinone 10 g/L,pH 7.5,30 °C and 72 h reaction time.(R)-3-Quinuclidinol is ob-tained with an yeild of 90% and with an optical purity of 88% enantiometric excess(ee) from a 100 mL reaction mixture.