Z. Wen-hui
2008
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Journal
Journal of Agro-Environment Science
Abstract
Aniline is a kind of important chemical material,it is also a harmful substance which pollutes environment and seriously endangers human health,and it has been included in "the blacklist of China for priority control in environment protection".Through domestication cultivation,a highly efficient aniline degrading bacterium strain was isolated from an activated mud drained from a chemical plant and named as strain AN5.It is gram positive,spherical or short rod-like,generally shows orange-red colony.Besides aniline,it can grow with phenol,benzoate,or naphthalene as unique carbon source.The isolated strain AN5 showed similarity of 99% to the 16S rDNA sequence of Rhodococcus pyridinivorans.The phylogenetic analysis was made on strain AN5 and the relative strains by applying maximum-likelihood(ML) methods with PAUP4.0 software.The optimum temperature and pH value for growth and degradation of strain AN5 were 30℃ and 7.0,respectively.Strain AN5 can grow in the medium containing aniline at the concentration up to 5 000 mg·L-1.The addition of peptone to medium containing aniline resulted in acceleration in the aniline degradation rate.Aniline was degraded by strain AN5 through ortho-cleavage pathway by catechol-1,2-dioxygenase.