M. Hamberg
Mar 27, 1984
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0
Influential Citations
2
Citations
Quality indicators
Journal
Biochimica et biophysica acta
Abstract
Synthesis of intramolecularly labeled 6,9,12-[11,11-2H2,1-14C]octadecatrienoic acid is described. The doubly labeled acid was added to 6,9,12-[9,10-3H2]octadecatrienoic acid and the mixture was incubated with soybean and human platelet lipoxygenases and was subjected to autoxidation. Methyl 13-hydroxyoctadecanoate obtained in the three sets of experiments had a considerably reduced 14C/3H ratio compared to that of the starting material, i.e., 20-24% (autoxidation) and 12-15% (lipoxygenase oxygenations). This indicates that subtraction of a hydrogen atom from the methylene group of the cis,cis-pentadiene structure is the rate-limiting step in autoxidation as well as in the enzymatic oxygenations.