S. Eremin, E. G. Matveeva, A. Gómez-Hens
Jul 1, 1998
Citations
0
Influential Citations
7
Citations
Journal
International Journal of Environmental Analytical Chemistry
Abstract
Abstract A kinetic metodology was applied to the determination of 2,4-dichlorophenoxyacetic acid (2,4-D) by using fluorescence polarization immunoassay (FPIA). The analytical parameter used was the initial rate of the antigen-antibody reaction which was obtained from the kinetic curve degree of polarization-time and measured in only 1 s. The stopped-flow mixing technique was used for this purpose which allows the application of the method to routine analysis. The dynamic range of the calibration graph is 10–1000 ng ml−1 and the detection limit is 4 ng ml−1, lower than that afforded by the conventional FPIA. The relative standard deviation was close to 4%. The method was directly applied to the analysis of river water, grape juice and white wine and the analytical recoveries ranged from 92 to 110%.