Yu Zhang, Xiaohui Di, Wen Wang
Sep 15, 2020
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Influential Citations
12
Citations
Journal
Biochemical Engineering Journal
Abstract
Abstract Methyl-2-furoate (MF) is a high-value compound, and can be produced via esterification of furoic acid derived from biobased furfural. Herein, CAL-B and [bmim][PF6] were the most efficient lipase and organic medium for MF production, respectively. Based on mass action law and reaction reversibility, a mathematical model was developed. Data analysis showed the newly proposed binary regression was apparently more suitable compared to the commonly used unitary regression. The enzymatic promotion factor mi for esterification and hydrolysis was 1.8159 and 0, while the alcohol inhibition factor ni was 0 and 0.06436, respectively. Both the activation energy Ea,i for esterification and hydrolysis was 84 kJ/mol, and the pre-exponential factor Ai was 7.1408×1013 and 7.8962×1013, respectively. Kinetics study demonstrated that CAL-B held significantly more dominant esterification activity compared to hydrolysis activity, whereas alcohol just inhibited hydrolysis activity instead of esterification activity, and the relationship between esterification/hydrolysis rate constants ki and temperature agreed with Arrhenius equation. The maximum MF yield was 82.5 % at 24 h, and the yield decreased to 75.4 % after 1 re-use of CAL-B, but maintained about 20 % for next 4 cycles. Totally, the developed model and binary regression analysis method is ideal for in-depth examination of lipase-catalyzed kinetics in heterogeneous system.