S. Tsukiji, M. Miyagawa, Yousuke Takaoka
Mar 29, 2009
Citations
1
Influential Citations
284
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Quality indicators
Journal
Nature chemical biology
Abstract
Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.