S. Allenmark, S. Andersson
1986
Citations
0
Influential Citations
39
Citations
Journal
Journal of Chromatography A
Abstract
Abstract Analytical-scale optical resolution of a series of N-(2,4-dinitrophenyl)- and dansyl- d , l -amino acids has been effected by the use of a bovine serum albumin silica column (Resolvosil). Decreasing retention was found for both types of amino acid with increasing pH or 1-propanol content of the mobile phase. In the dinitrophenyl series, the aspartic acid derivatives showed very large enantiomeric separation factors compared with the glutamic acid homologue, and an analogous, but less pronounced, efffect was found for the phenylglycine—phenylalanine pair. Fluorimetric studies of dansyl-alanine showed that by a simple post-column addition of 1-propanol the fluorescence yield can be increased by a factor of over 20,giving a very low detection limit. The usefulness of the analytical technique for the determination of the bacterial marker compounds, d -alanine and d -glutamic acid, present in cell wall hydrolysates, is emphasized.