T. Rothstein, J. Blum
Jul 1, 1974
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0
Influential Citations
16
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Quality indicators
Journal
Experimental cell research
Abstract
Abstract Homogenates of control cells prior to and after incubation for 2.5 h in dilute salt solution were sedimented on sucrose density gradients, as were cells incubated for 2.5 h with dichloroisoproterenol. In control cells, acid phosphatase and ribonuclease were located separately in two different high density particles; a third, low density, particle contained both hydrolase activities. Starvation (with concomitant extracellular acid hydrolase release) induced the specific loss of the high density particles, but the presence of dichloroisoproterenol partially reversed this trend. Sedimentation studies also showed that ingested dye particles were located in a high density particle which did not co-sediment with either acid phosphatase or ribonuclease.