T. Osawa
Mar 1, 1966
Citations
0
Influential Citations
53
Citations
Journal
Carbohydrate Research
Abstract
Abstract In order to study the substrate specificities of egg-white lysozyme, p -nitrophenyl 2-acetamido-4- O -(2-acetamido-2-deoxy-β- D -glucopyranosyl)-2-deoxy-β- D -glucopyranoside (V) and p -nitrophenyl 2-acetamido-6- O -(2-acetamido-2-deoxy-β- D -glucopyranosyl)-2-deoxy-β- D -glucopyranoside (XI) were synthesized. Compound V was hydrolyzed by lysozyme, even though its hydrolysis was slow and required relatively massive proportions of the enzyme. On the other hand, both XI and p -nitrophenyl 2-acetamido-2-deoxy-β- D -glucopyranoside (VI) were found to be unaffected by the enzyme. These observations are discussed in relation to the structure of a disaccharide isolated from the cell wall of Micrococcus lysodeikticus by the action of lysozyme, and to the mechanism of resistance of certain bacteria to egg-white lysozyme.