K. Schosinsky, H Peter Lehmann, M. Beeler
Dec 1, 1974
Citations
14
Influential Citations
587
Citations
Quality indicators
Journal
Clinical chemistry
Abstract
We have developed a new method for determination of serum ceruloplasmin from its oxidase activity, in which o -dianisidine dihydrochloride is used as substrate. o -Dianisidine dihydrochloride is more stable than is the more widely used p -phenylenediamine substrate, and forms a stable product that is measured at 540 nm. Correlation was good between results of this method and those obtained with both a p -phenylenediamine substrate method ( r = 0.98) and a radial immunodiffusion procedure ( r = 0.97). There is little or no interference from reducing or colored components of serum. The coefficient of variation (day-to-day) for the method was 4.2%. A normal range of 62-140 U/ liter has been determined by the reported method.