D. Sandberg, C. Z. Bacallao, W. Cleveland
Dec 1, 1970
Citations
2
Influential Citations
29
Citations
Journal
Biochemical medicine
Abstract
Abstract Although recently procedures have been developed for measurement of prednisolone (11β,17α,21-trihydroxypregna-1,2-diene-3,20-dione) in blood, they lack sensitivity needed for plasma concentrations found in patients on usual therapeutic dosages (1, 2). We have applied the competitive protein-binding radioassay technique developed by Murphy to measurement of plasma prednisolone (3). This method, while very sensitive, also contributes considerable specificity due to the high affinity of the protein used for binding (4). Preliminary purification is provided by extraction with methylene chloride and paper chromatographic separation of prednisolone from cortisol (11β,17α,21-trihydroxypregna-4-ene-3,20-dione) using a Bush descending chromatographic system (5). The assay is performed by displacement of tritiated corticosterone (11,21-dihydroxypregna-4-ene-3,20-dione) from corticosterone-binding globin (CBG) present in dog plasma. The specific technique used is a modification of one for measurement of progesterone published by Neill and his co-workers (4).