L. C. Crowley, B. Marfell, M. Christensen
Jul 1, 2016
Citations
3
Influential Citations
71
Citations
Quality indicators
Journal
Cold Spring Harbor protocols
Abstract
Trypan blue is a colorimetric dye that stains dead cells with a blue color easily observed using light microscopy at low resolution. The staining procedure is rapid and cells can be analyzed within minutes. The number of live (unstained) and dead (blue) cells can be counted using a hemocytometer on a basic upright microscope. Trypan blue staining is therefore a convenient assay for rapidly determining the overall viability of cells in a culture before commencing scientific experimentation, or for quantitating cell death following treatment with any cytotoxic stimuli.