Zhenyu Zhang, Pengfu Liu, Weike Su
Apr 21, 2021
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Influential Citations
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Journal
Microbial Cell Factories
Abstract
Trans- 4-hydroxy- l -proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans- 4-hydroxy- l -proline is produced by the acidic hydrolysis of collagen, but this process has serious drawbacks, such as low productivity, a complex process and heavy environmental pollution. Presently, trans- 4-hydroxy- l -proline is mainly produced via fermentative production by microorganisms. Some recently published advances in metabolic engineering have been used to effectively construct microbial cell factories that have improved the trans- 4-hydroxy- l -proline biosynthetic pathway. To probe the potential of microorganisms for trans -4-hydroxy- l -proline production, new strategies and tools must be proposed. In this review, we provide a comprehensive understanding of trans -4-hydroxy- l -proline, including its biosynthetic pathway, proline hydroxylases and production by metabolic engineering, with a focus on improving its production.